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National Cheng Kung University

16 ARTICLES PUBLISHED IN JoVE

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Education

A Fabrication Method for Highly Stretchable Conductors with Silver Nanowires
Chia-Wei Chang 1, Shih-Pin Chen 1, Ying-Chih Liao 1
1Department of Chemical Engineering, National Taiwan University

A simple synthesis method is used to chemically solder silver nanowire thin film to fabricate highly stretchable and conductive metal conductors.

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Immunology and Infection

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C)
Ke-Huan Chow *1, Zihao Yan *1,2, Wei-Li Wu 1
1Division of Biology and Biological Engineering, California Institute of Technology, 2Harvard Medical School

Maternal immune activation (MIA) is a model for an environmental risk factor of autism and schizophrenia. The goal of this article is to provide a step-by-step procedure of how to induce MIA in the pregnant mice in order to enhance the reproducibility of this model.

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Behavior

An Innovative Running Wheel-based Mechanism for Improved Rat Training Performance
Chi-Chun Chen 1, Chin-Lung Yang 2, Ching-Ping Chang 3,4
1Department of Electronic Engineering, National Chin-Yi University of Technology, 2Department of Electrical Engineering, National Cheng Kung University, 3Department of Biotechnology, Southern Taiwan University of Science and Technology, 4Department of Medical Research, Chi Mei Medical Center

This study presents an innovative running wheel-based animal mobility system to quantify an effective exercise activity in rats. A rat-friendly testbed is built, using a predefined adaptive acceleration curve, and a high correlation between the effective exercise rate and the infarct volume suggests the protocol's potential for stroke prevention experiments.

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Immunology and Infection

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
Cheng-Ju Kuo 1,2, Sin-Tian Wang 1,2, Chang-Shi Chen 1,2
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.

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Cancer Research

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
Tsung-Cheng Lin 1, Ying-Chih Liao 2, Wen-Tsan Chang 1,2, Cheng-Han Yang 1, Li-Hsin Cheng 1, Megan Cheng 3, Hung-Chi Cheng 1,2
1The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 3Trauma Office, Children's National Health System

An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.

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Education

Uncoupling Coriolis Force and Rotating Buoyancy Effects on Full-Field Heat Transfer Properties of a Rotating Channel
Shyy Woei Chang 1, Wei-Ling Cai 1, Hong-Da Shen 1, Kuo-Ching Yu 1
1Department of System and Naval Mechatronic Engineering, National Cheng Kung University

Here, we present an experimental method for decoupling the interdependent Coriolis-force and rotating-buoyancy effects on full-field heat transfer distributions of a rotating channel.

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Immunology and Infection

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
Yi-Wei Chen 1, Wen-Chien Ko 1,2, Chang-Shi Chen 1,3, Po-Lin Chen 2,4
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University

Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species.

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Biology

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Yung-Chia Chen 1,2, Bu-Miin Huang 3
1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 2Department of Anatomy, School of Medicine, Kaohsiung Medical University, 3Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

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Immunology and Infection

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Ting-Jing Shen 1,2, Ming-Kai Jhan 1,2, Jo-Chi Kao 1,2, Min-Ru Ho 1,2, Tsung-Ting Tsai 1,2, Po-Chun Tseng 1,2, Yung-Ting Wang 1,2, Chiou-Feng Lin 1,2,3
1Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 2Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, 3Center of Infectious Disease and Signaling Research, National Cheng Kung University

Here, we present a protocol for creating an immunocompetent ICR (Institute of Cancer Research) murine model of central nervous system infection to display the development of neuropathy. Monitoring acute viral encephalitic disorders by identical disease scores could be performed for showing dengue virus-induced neuropathy in vivo.

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Behavior

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature
Yun Chang 1, Alan Ewert 2, Lisa M. Kamendulis 3, Barbara A. Hocevar 3
1School of Kinesiology and Recreation, Illinois State University, 2Department of Recreation, Park, and Tourism Studies, Indiana University, 3Department pf Environmental and Occupational Health, School of Public Health-Bloomington, Indiana University

The purpose of this paper is to identify changes in stress levels after visitation to three different settings and to describe the methods used in identifying stress levels based on measures of salivary cortisol, α-amylase, and a psychological self-report instrument. 

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Immunology and Infection

Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans
Jia-Horung Hung *1,2, Zhao-Xiang Wang *3,4, Yuan-Hsin Lo 5, Chaw-Ning Lee 3,6, Yun Chang 7, Rita Yuchiao Chang 7, Chien-Chun Huang 3, Tak-Wah Wong 3,8,9
1Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 2Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 5Department of Dermatology, Fu Jen Catholic University Hospital, Fu Jen Catholic University, 6Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, 7School of Medicine, National Cheng Kung University, 8Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 9Center of Applied Nanomedicine, National Cheng Kung University

The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro.

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Medicine

A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage
Min-Yu Chang *1,2, Hsi-Hao Wang *1,2, Li-Hung Chen *1, Jhen Gao *1, Shih-Yuan Hung 1,2, Yuan-Yow Chiou 3,4, Yi-Che Lee 1,2,5
1School of Medicine, College of Medicine, I-Shou University, 2Division of Nephrology, Department of Internal Medicine, E-DA Hospital, 3Department of Pediatrics, National Cheng Kung University Hospital, 4Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 5Division of Nephrology, Department of Internal Medicine, E-DA Dachang Hospital

The present protocol establishes a peritoneal dialysis (PD) mouse model of chlorhexidine gluconate (CG)-induced peritoneal fibrosis. The current model is simple and easy to use compared to other PD animal models.

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Neuroscience

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
Chia-Wei Liou *1,2, Tzu-Hsuan Yao *2, Wei-Li Wu 1,2
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Physiology, College of Medicine, National Cheng Kung University

Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

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Biology

Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing
Szu-Ying Chen 1,2, Po-Hsien Huang 1,2
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

This protocol provides an easy-to-follow workflow to conduct poly(A) RNA purification, bisulfite conversion, and library preparation using standardized equipment for a biological sample of interest.

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Bioengineering

Finite Element Analysis Model for Assessing Expansion Patterns from Surgically Assisted Rapid Palatal Expansion
Jia-Hong Lin *1, Guan-Lin Wu *2, Chun-Kai Chiu 2, Steven Wang 3, Chun-Hsi Chung 1, Chenshuang Li 1
1Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, 2Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, 3Department of Oral and Maxillofacial Surgery/Pharmacology, School of Dental Medicine, University of Pennsylvania

A set of novel finite element models of surgically assisted rapid palatal expansion (SARPE) that could perform a clinically required amount of expander activation with various angles of buccal osteotomy was created for further analysis of the expansion patterns of the hemimaxillae in all three dimensions.

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Biology

Induction of Petite Colonies in Candida glabrate via Rose Bengal-Mediated Photodynamic Therapy
Cai-Ying Yang *1,2, Jia-Horung Hung *3, Chi-Jung Wu 4,5, Zhao-Xiang Wang 1,2, Shih-Han Wang 6, Hao-Chun Liaw 2, I-Huang Lin 3, Chun-Keung Yu 1,6, Tak-Wah Wong 2,7,8
1Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 2Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 4National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, 5Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 6Insititute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 7Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 8Center of Applied Nanomedicine, National Cheng Kung University

The significance of petite colonies in Candida spp. drug resistance has not been fully explored. Antimicrobial photodynamic therapy (aPDT) offers a promising strategy against drug-resistant fungal infections. This study demonstrates that rose bengal-mediated aPDT effectively deactivates Candida glabrata and induces petite colonies, presenting a unique procedure.

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