Establishing animal model for peritoneal dialysis, PD, is very important. We hope it can improve the technology and the efficacy of PD therapy and eventually improve the patient's survival. This peritoneal dialysis mouse model is simple, easy to use, and visible compared to other peritoneal dialysis animal models.
A suitable peritoneal dialysis animal model can help researchers to study the peritoneal, physiological, and the pathological change during PD therapy. Demonstration of the procedure will be Fang-Ling Liao, a research assistant from my laboratory. To begin, prepare the chemical irritant by diluting 0.1%chlorhexidine gluconate in 15%ethanol.
Next, assign three mice as the control group and inject one milliliter per kilogram of 0.9%normal saline intraperitoneally every other day for three weeks for a total of nine times. Then assign three mice to the peritoneal fibrosis group, and induce peritoneal fibrosis by administering an intraperitoneal injection of 0.1%chlorhexidine gluconate solution prepared earlier at a dose of 12.5 microliters per kilogram of the body weight. For peritoneal function tests, prepare a dialysis solution containing 4.25%glucose.
Then use a syringe to draw 0.5 milliliters of dialysate sample. Prepare an injection of zoletil and xylazine and anesthetize the mice by administering an intramuscular injection. Perform intraperitoneal installation of the dialysis solution at a dose of two milliliters per 20 grams of body weight.
Make a small incision in the midline of the abdomen and collect the major intraperitoneal fluid with a syringe. After 30 minutes, perform a vertical incision in the midline of the abdomen beneath the xiphoid process and open the mouse's abdomen to collect the residual intraperitoneal fluid with a syringe. Next, measure the weight of a clean and dry cotton swab.
Put the cotton into the mouse's abdominal cavity to absorb the residual intraperitoneal fluid and then remove it. Measure the cotton weight again. Masson's trichrome staining showed that the parietal peritoneum of the abdominal wall in the chlorhexidine gluconate-exposed group was markedly thicker and fibrotic than the control group.
The visceral peritoneum of the liver surfaces was also significantly thicker and fibrotic in the chlorhexidine gluconate-exposed group than in the control saline group, indicating that peritoneal fibrosis is more severe in the chlorhexidine gluconate-exposed group. The chlorhexidine gluconate-exposed group showed decreased ultrafiltration rate and increased glucose mass transport, indicating that the peritoneal permeability increases in the chlorhexidine gluconate exposed mice While performing an intraperitoneal injection procedure, use the forceps and pick the abdominal skin of the mouse to prevent puncture-induced intraperitoneal organ damage.
