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National Taipei University of Technology

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Preparation of Homogeneous MALDI Samples for Quantitative Applications
Yu-Meng Ou 1,2, Chien-Wei Tsao 1,3, Yin-Hung Lai 1, Hsun Lee 1, Huan-Tsung Chang 2, Yi-Sheng Wang 1
1Genomic Research Center, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology

A protocol for reducing spatial heterogeneities of ion signals in MALDI mass spectrometry by regulating substrate temperature during sample drying processes is demonstrated.

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Bioengineering

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
Hua-De Gao 1,2, Pounraj Thanasekaran 1, Tzu-Ho Chen 1,2, Yu-Hsu Chang 3, Yu-Ju Chen 1,2, Hsien-Ming Lee 1
1Institute of Chemistry, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Materials and Mineral Resources Engineering, National Taipei University of Technology

In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.

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Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
Chun Hsiung *1,2, Yu-Ting Liu *1,2, Chen-Ying Su 3, Chung-Chuan Hsiung 4,5, Kuo-Hsuan Hung 2,6,7, Lung-Kun Yeh 2,6
1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

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