An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
7.5K Views
•
11:20 min
•
August 30th, 2017
DOI :
August 30th, 2017
•Chapters in this video
0:05
Title
1:33
Fluorimeter Setup for Upconversion Spectrum Measurement and Microscope Setup
3:29
Characterization of Caged-PKA and Caged PKA/UCNP Constructs and Photolysis Setup
4:55
Cell Sample Preparation and Microinjection of Caged-PKA/UCNP Complexes
7:35
Photoactivation of Caged-PKA/UCNP Using NIR Light in Living Cells Experiment
8:33
Results: Visualize PKA Signal Transduction Activation via NIR Photoactivation
9:40
Conclusion
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