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University of Verona

6 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
Elisa Gecchele *1, Matilde Merlin *1, Annalisa Brozzetti 2, Alberto Falorni 2, Mario Pezzotti 1, Linda Avesani 1
1Department of Biotechnology, University of Verona, Verona, Italy, 2Department of Internal Medicine, University of Perugia, Perugia, Italy

In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.

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Genetics

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Silvia Dal Santo *1, Mauro Commisso *1, Erica D'Incà 1, Andrea Anesi 1,2, Matteo Stocchero 3, Sara Zenoni 1, Stefania Ceoldo 1, Giovanni B. Tornielli 1, Mario Pezzotti 1, Flavia Guzzo 1
1Biotechnology Department, University of Verona, 2Lab. of Bioorganic Chemistry, Physics Department, University of Trento, 3S-IN Soluzioni Informatiche

This article describes the application of untargeted metabolomics, transcriptomics and multivariate statistical analysis to grape berry transcripts and metabolites in order to gain insight into the terroir concept, i.e., the impact of the environment on berry quality traits.

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Immunology and Infection

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
Gabriella Bergamini *1, Fabio Stellari *2, Angela Sandri *3, Maria M. Lleo 3, Gaetano Donofrio 4, Francesca Ruscitti 5,2, Federico Boschi 6, Andrea Sbarbati 7, Gino Villetti 2, Paola Melotti 8, Claudio Sorio 1
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona

The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.

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Bioengineering

Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes
Mattia Santoni *1, Edoardo Bertini *1, Roberta Zampieri 1, Anna Cuccurullo 1, Mauro Commisso 1, Elisa Gecchele 1, Linda Avesani 1
1Department of Biotechnology, University of Verona

Here, we present a protocol to produce an oral vaccine candidate against Type 1 diabetes in an edible plant.

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Immunology and Infection

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay
Tobia Fantoni *1, Michele Bissoli *1, Chiara Stefani 1, Mauro Voi 1, Alexandrina Dabija 1, Rebecca Casula 1, Domenico Luca Minafra 1, Julys da Fonseca Palmeira 2, Enrique Roberto Argañaraz 2, Martin Mayora-Neto 3, Nigel J. Temperton 3, Donato Zipeto 1, Alessandra Ruggiero 1
1Department of Neuroscience, Biomedicine and Movement, University of Verona, 2Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, 3Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway

Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients' serum after COVID-19 vaccination.

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Medicine

Clinical Anthropometrics and Body Composition from 3-Dimensional Optical Imaging
Marco A. Minetto 1, Chiara Busso 1, Andrea Ferraris 1, Angelo Pietrobelli 2,3, John A. Shepherd 4, Cassidy McCarthy 2, Steven B. Heymsfield 2
1Division of Physical Medicine and Rehabilitation, Department of Surgical Sciences, University of Turin, 2Pennington Biomedical Research Centre, 3Department of Surgical Sciences, Dentistry, Gynaecology and Paediatrics, Paediatric Unit, University of Verona, 4Department of Epidemiology, University of Hawaii Cancer Center

The procedures for body size, shape, and composition assessment through commercially available solutions for three-dimensional optical imaging enable the rapid collection of accurate and reproducible data. Clinicians could implement the acquisition of innovative and useful biomarkers ("e-tape" measurements) in routine evaluations of patients to assist in characterizing their health status.

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