An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

7.3K Views

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08:16 min

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July 7th, 2017

DOI :

10.3791/55499-v

July 7th, 2017

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Gabriella Bergamini*¹, Fabio Stellari*², Angela Sandri*³, Maria M. Lleo³, Gaetano Donofrio⁴, Francesca Ruscitti⁵^,², Federico Boschi⁶, Andrea Sbarbati⁷, Gino Villetti², Paola Melotti⁸, Claudio Sorio¹

¹Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, ²Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., ³Department of Diagnostic and Public Health, Microbiology Division, University of Verona, ⁴Dipartimento di Scienze Medico-Veterinarie, University of Parma, ⁵Department of Biomedical Biotechnological and Translational Sciences, University of Parma, ⁶Department of Computer Science, University of Verona, ⁷Department of Neurological, Biomedical and Movement Sciences, University of Verona, ⁸Cystic Fibrosis Regional Center (CFC), AOUI Verona

Summary

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Keyword Extraction IL 8

In Vivo

Lung Inflammation

Bacterial Culture Supernatant

Respiratory Infection

Gene Delivery

Bioluminescent Imaging

D Luciferin

In Vivo Imaging System

Luminescent Imaging Mode

Photons

Chapters in this video

0:05

Title

1:07

In Vivo Gene Delivery and Biomluminescent Imaging (BLI) and Photon Quantitification

4:24

Pro-inflammatory Stimuli Mouse Challenge

6:51

Results: Representative P. aeruginosa-induced Lung Inflammation Supernatent In Vivo Imaging in IL-8 Transiently Trangenic Mice

7:42

Conclusion

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