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National Central University

3 ARTICLES PUBLISHED IN JoVE

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Engineering

Effect of Bending on the Electrical Characteristics of Flexible Organic Single Crystal-based Field-effect Transistors
Man-Tzu Ho 1,2, Yu-Tai Tao 1
1Institution of Chemistry, Academia Sinica, 2Department of Chemistry, National Central University

This manuscript describes the bending process of an organic single crystal-based field-effect transistor to maintain a functioning device for electronic property measurement. The results suggest that bending causes changes in the molecular spacing in the crystal and thus in the charge hopping rate, which is important in flexible electronics.

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Bioengineering

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
Tzu-Cheng Sung *1, Hsing-Fen Li *1, Akon Higuchi 1,2, Qing-Dong Ling 3,4, Jia-Sin Yang 1, Yeh-Chia Tseng 1, Chih-Hsien Pan Pan 1, Abdullah A. Alarfaj 2, Murugan A. Munusamy 2, Suresh Kumar 5, Shih-Tien Hsu 6, Kadarkarai Murugan 7,8
1Department of Chemical and Materials Engineering, National Central University, 2Department of Botany and Microbiology, King Saud University, 3Cathay Medical Research Institute, Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, 5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 6Department of Internal Medicine, Taiwan Landseed Hospital, 7Department of Zoology, Bharathiar University, 8Thiruvalluvar University

A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time.

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Biochemistry

Preparation of High-Temperature Sample Grids for Cryo-EM
Yuan-Chih Chang 1,2, Chin-Yu Chen 3,4, Ming-Daw Tsai 1,5
1Institute of Biological Chemistry, Academia Sinica, 2Academia Sinica Cryo-EM Facility, Academia Sinica, 3Department of Life Sciences, National Central University, 4Hubei University, 5Institute of Biochemical Sciences, National Taiwan University

This paper provides a detailed protocol for preparing sample grids at temperatures as high as 70 °C, prior to plunge freezing for cryo-EM experiments.

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