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Preparation of High-Temperature Sample Grids for Cryo-EM

DOI :

10.3791/62772-v

July 26th, 2021

July 26th, 2021

3,118 Views

1Institute of Biological Chemistry, Academia Sinica, 2Academia Sinica Cryo-EM Facility, Academia Sinica, 3Department of Life Sciences, National Central University, 4Hubei University, 5Institute of Biochemical Sciences, National Taiwan University

This paper provides a detailed protocol for preparing sample grids at temperatures as high as 70 °C, prior to plunge freezing for cryo-EM experiments.

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High Temperature Sample Grids

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