University of California in San Diego School of Medicine and Howard Hughes Medical Institute

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

¹Division of Biological Sciences, Section of Neurobiology, University of California in San Diego and Howard Hughes Medical Institute, ²Department of Cellular and Molecular Medicine, University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Genetically-encoded histone-miniSOG induces genome-wide heritable mutations in a blue light-dependent manner. This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration.