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Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

DOI :

10.3791/54810-v

November 14th, 2016

November 14th, 2016

8,786 Views

1Division of Biological Sciences, Section of Neurobiology, University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine, University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Genetically-encoded histone-miniSOG induces genome-wide heritable mutations in a blue light-dependent manner. This mutagenesis method is simple, fast, free of toxic chemicals, and well-suited for forward genetic screening and transgene integration.

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