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University of Florida College of Medicine

6 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Endobronchial Ultrasound-guided Intratumoral Injection of Cisplatin for the Treatment of Isolated Mediastinal Recurrence of Lung Cancer
Hiren J. Mehta 1, Michael A. Jantz 1
1Division of Pulmonary/Critical care/Sleep Medicine, University of Florida College of Medicine

The management of isolated recurrent lung cancer in a previously-irradiated field is challenging. Here, we describe an endobronchial ultrasound (EBUS)-guided cisplatin injection for the management of patients with localized lung cancer recurrence in a previously-radiated field.

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Cancer Research

Assays for Validating Histone Acetyltransferase Inhibitors
Aaron R. Waddell 1, Daiqing Liao 1
1Department of Anatomy and Cell Biology, and UF Health Cancer Center, University of Florida College of Medicine

Inhibitors of histone acetyltransferases (HATs, also known as lysine acetyltransferases), such as CBP/p300, are potential therapeutics for treating cancer. However, rigorous methods for validating these inhibitors are needed. Three in vitro methods for validation include HAT assays with recombinant acetyltransferases, immunoblotting for histone acetylation in cell culture, and ChIP-qPCR.

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Neuroscience

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
Jose A. Pino *1, Nora Awadallah *2, Alessandra M. Norris 3, Gonzalo E. Torres 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

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Developmental Biology

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
Connor D. Johnson 1, Lylybell Y. Zhou 1, Daniel Kopinke 1,2
1Department of Pharmacology and Therapeutics, University of Florida College of Medicine, 2Myology Institute, University of Florida College of Medicine

The replacement of healthy muscle tissue with intramuscular fat is a prominent feature of human diseases and conditions. This protocol outlines how to visualize, image, and quantify intramuscular fat, allowing the rigorous study of the mechanisms underlying intramuscular fat formation.

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Medicine

Micro-Ultrasound Guided Transperineal Prostate Biopsy: A Clinic-Based Procedure
Rani Ashouri 1, Brianna Nguyen 1, Jeremy Archer 2, Paul Crispen 1, Padraic O’Malley 1, Li-Ming Su 1, Joseph Grajo 1,3, Sara M. Falzarano 4, Yahya Acar 5,6,7, David Lizdas 5,6, Samsun Lampotang 1,5,6, Wayne Brisbane 1,6
1Department of Urology, University of Florida College of Medicine, 2Division of Urology, North Mississippi Medical Center, 3Department of Radiology, University of Florida College of Medicine, 4Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, 5Department of Anesthesiology, University of Florida College of Medicine, 6Center for Safety, Simulation & Advanced Learning Technologies, University of Florida, 7Department of Emergency Medicine, Gulhane School of Medicine

This manuscript outlines the steps for performing a micro-ultrasound-guided transperineal prostate biopsy under local anesthesia.

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Biology

Advanced Abdominal Aortic Aneurysm Modeling in Mice by Combination of Topical Elastase and Oral ß-aminopropionitrile
Jack Bontekoe 1, Gilbert Upchurch 2, Courtney Morgan 1, Bo Liu 3
1Division of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison School of Medicine and Public Health, 2Division of Vascular Surgery and Endovascular Therapy, University of Florida College of Medicine, 3Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health

This protocol describes a methodical surgical approach to modeling advanced abdominal aortic aneurysms in mice by a combination of directly applying elastase to the infrarenal aorta and administering ß-aminopropionitrile through drinking water.

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