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Assays for Validating Histone Acetyltransferase Inhibitors

DOI :

10.3791/61289-v

August 6th, 2020

August 6th, 2020

6,225 Views

1Department of Anatomy and Cell Biology, and UF Health Cancer Center, University of Florida College of Medicine

Inhibitors of histone acetyltransferases (HATs, also known as lysine acetyltransferases), such as CBP/p300, are potential therapeutics for treating cancer. However, rigorous methods for validating these inhibitors are needed. Three in vitro methods for validation include HAT assays with recombinant acetyltransferases, immunoblotting for histone acetylation in cell culture, and ChIP-qPCR.

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