Sanofi Pasteur Limited

2 ARTICLES PUBLISHED IN JoVE

Medicine

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Takashi Shingu¹, Mariela Jaskelioff², Liang Yuan¹, Zhihu Ding³, Alexei Protopopov⁴, Maria Kost-Alimova⁴, Jian Hu¹

¹Department of Cancer Biology, UT MD Anderson Cancer Center, ²Novartis Institutes for Biomedical Research, ³Sanofi US, ⁴Institute of Applied Cancer Science, UT MD Anderson Cancer Center

Telomere and telomerase play essential roles in ageing and tumorigenesis. The goal of this protocol is to show how to generate two murine inducible telomerase knock-in alleles and how to utilize them in the studies of tissue degeneration/regeneration and cancer.

JoVE Core

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Ibrahim B. Durowoju¹, Kamaljit S. Bhandal¹, Jian Hu¹, Bruce Carpick¹, Marina Kirkitadze¹

¹Analytical Research & Development, Sanofi Pasteur Limited

Differential scanning calorimetry measures the thermal transition temperature(s) and total heat energy required to denature a protein. Results obtained are used to assess the thermal stability of protein antigens in vaccine formulations.