German Cancer Consortium (DKTK)

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

Susan Kochall¹, May-Linn Thepkaysone¹, Sebastián A. García¹, Alexander M. Betzler¹, Jürgen Weitz^1,2,3, Christoph Reissfelder¹, Sebastian Schölch^1,2,3

¹Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, ²German Cancer Consortium (DKTK), ³German Cancer Research Center (DKFZ)

We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

Cancer Research

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Alexander M. Betzler¹, Susan Kochall¹, Linda Blickensdörfer², Sebastian A. Garcia¹, May-Linn Thepkaysone¹, Lahiri K. Nanduri¹, Michael H. Muders³, Jürgen Weitz^1,4,5, Christoph Reissfelder¹, Sebastian Schölch^1,4,5

¹Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, ²Department of General, Gastrointestinal and Transplant Surgery, University of Heidelberg, ³Department of Pathology, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, ⁴German Cancer Consortium (DKTK), ⁵German Cancer Research Center (DKFZ)

A protocol for the establishment of a genetically engineered mouse model of colorectal cancer by segmental adeno-cre infection and its surveillance via high-resolution colonoscopy is presented.

Genetics

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Anne-Marlen Gaebler¹, Alexander Hennig¹, Katharina Buczolich¹, Jürgen Weitz¹, Thilo Welsch¹, Daniel E. Stange¹, Kristin Pape¹

¹Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden

This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation buffers.