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German Cancer Consortium (DKTK)

3 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
Susan Kochall 1, May-Linn Thepkaysone 1, Sebastián A. García 1, Alexander M. Betzler 1, Jürgen Weitz 1,2,3, Christoph Reissfelder 1, Sebastian Schölch 1,2,3
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

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Cancer Research

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
Alexander M. Betzler 1, Susan Kochall 1, Linda Blickensdörfer 2, Sebastian A. Garcia 1, May-Linn Thepkaysone 1, Lahiri K. Nanduri 1, Michael H. Muders 3, Jürgen Weitz 1,4,5, Christoph Reissfelder 1, Sebastian Schölch 1,4,5
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 2Department of General, Gastrointestinal and Transplant Surgery, University of Heidelberg, 3Department of Pathology, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 4German Cancer Consortium (DKTK), 5German Cancer Research Center (DKFZ)

A protocol for the establishment of a genetically engineered mouse model of colorectal cancer by segmental adeno-cre infection and its surveillance via high-resolution colonoscopy is presented.

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Genetics

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
Anne-Marlen Gaebler 1, Alexander Hennig 1, Katharina Buczolich 1, Jürgen Weitz 1, Thilo Welsch 1, Daniel E. Stange 1, Kristin Pape 1
1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden

This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation buffers.

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