University Medical Center Hamburg-Eppendorf and DZHK (German Center for Cardiovascular Research)

4 ARTICLES PUBLISHED IN JoVE

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Lenard Conradi^1,2, Christiane Pahrmann¹, Stephanie Schmidt¹, Tobias Deuse^1,3, Arne Hansen², Alexandra Eder², Hermann Reichenspurner¹, Robert C. Robbins³, Thomas Eschenhagen², Sonja Schrepfer^1,3

¹Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, ²Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, ³CT Surgery, Stanford University School of Medicine

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

JoVE Core

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Ingra Mannhardt¹, Umber Saleem¹, Anika Benzin¹, Thomas Schulze¹, Birgit Klampe¹, Thomas Eschenhagen¹, Arne Hansen¹

¹Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf and DZHK (German Center for Cardiovascular Research)

Here, we show the generation of human engineered heart tissue from induced pluripotent stem cells (hiPSC)-derived cardiomyocytes. We present a method to analyze contraction force and exemplary alteration of contraction pattern by the hERG channel inhibitor E-4031. This method shows high level of robustness and suitability for cardiac drug screening.

Medicine

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

Liesa Castro^1,2, Birgit Geertz³, Marina Reinsch^2,3, Bülent Aksehirlioglu³, Arne Hansen^2,3, Thomas Eschenhagen^2,3, Hermann Reichenspurner^1,2, Florian Weinberger^*2,3, Simon Pecha^*1,2

¹Department of Cardiovascular Surgery, University Heart Center Hamburg, ²partner site Hamburg/Kiel/Lübeck, German Centre for Cardiovascular Research (DZHK), ³Department of Experimental Pharmacology and Toxicology, Cardiovascular ResearchCenter, University Medical Center Hamburg-Eppendorf

Here we present a protocol for the induction of left ventricular cryoinjury followed by the implantation of a cardiac muscle patch, derived from human iPS-cell cardiomyocytes in a guinea pig model.

Developmental Biology

Magnetic Adjustment of Afterload in Engineered Heart Tissues

Benjamin Becker^*1,2, Marita L. Rodriguez^*1,2, Tessa R. Werner^1,2, Justus Stenzig^1,2, Thomas Eschenhagen^1,2, Marc N. Hirt^1,2

¹Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, ²DZHK (German Centre for Cardiovascular Research)

This protocol provides detailed methods describing the fabrication and implementation of a magnetics-based afterload tuning platform for engineered heart tissues.