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Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

9.6K Views

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07:33 min

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June 1st, 2011

DOI :

10.3791/2605-v

June 1st, 2011

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1Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, 2Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, 3CT Surgery, Stanford University School of Medicine

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Bioluminescence Imaging
Assessment
Immune Responses
Implantation
Engineered Heart Tissue
Cardiac Tissue Engineering
Scaffolding Strategies
Native Scaffold Materials
Bioartificial Scaffold Materials
Cardiac Myocytes
Hydrogels
Fibrin
Collagen
Myocyte Monolayers
Restoration Of Cardiac Function
Myocardial Infarction
Experimental Models
Predictive Toxicology
Substance Screening
Disease Modelling
Cell Survival Monitoring
In vivo Bioluminescence Imaging BLI Techniques
Fibrin based EHT Generation
Transgenic Rat Strain
Firefly Luciferase Expression
ROSA luciferase LEW Tg Strain
Omentum Implantation
Immune Responses Assessment
BLI Results Comparison With ELISPOT Technique

Chapters in this video

0:05

Title

1:26

EHT Generation and Culture Conditions

3:00

EHT Implantation

4:57

Bioluminescence Imaging of EHT following Implantation

6:08

Correlation of BLI Results and ELISPOT

7:11

Conclusion

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