Ananda Devices

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection

Margaret H. Magdesian^1,2,3, Madeleine Anthonisen¹, G. Monserratt Lopez-Ayon¹, Xue Ying Chua¹, Matthew Rigby¹, Peter Grütter¹

¹Department of Physics, McGill University, ²Department of Neurology and Neurosurgery, Montreal Neurological Institute, ³Ananda Devices

This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.