Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal ConnectionMargaret H. Magdesian 1,2,3, Madeleine Anthonisen 1, G. Monserratt Lopez-Ayon 1, Xue Ying Chua 1, Matthew Rigby 1, Peter Grütter 1
1Department of Physics, McGill University, 2Department of Neurology and Neurosurgery, Montreal Neurological Institute, 3Ananda Devices
This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.
