Lethbridge Laboratories

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Yo Ching Cheng^*1, Samia Hannaoui^*1, Theodore Ralph John², Sandor Dudas³, Stefanie Czub³, Sabine Gilch¹

¹Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine, University of Calgary, ²Dept. of Molecular Biology, University of Wyoming, ³Canadian Food Inspection Agency, Lethbridge Laboratories

Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.