Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal MaterialYo Ching Cheng *1, Samia Hannaoui *1, Theodore Ralph John 2, Sandor Dudas 3, Stefanie Czub 3, Sabine Gilch 1
1Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine, University of Calgary, 2Dept. of Molecular Biology, University of Wyoming, 3Canadian Food Inspection Agency, Lethbridge Laboratories
Here, we present a protocol to describe a simple, fast and efficient prion amplification technique, the real-time quaking-induced conversion (RT-QuIC) method.
