Université de Namur

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Jean-Michel Vandeweerd¹, Fanny Hontoir¹, Alexis De Knoop¹, Kathleen De Swert², Charles Nicaise²

¹URVI, NARILIS, Université de Namur, ²URPhyM, NARILIS, Université de Namur

Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.