Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in MiceJean-Michel Vandeweerd 1, Fanny Hontoir 1, Alexis De Knoop 1, Kathleen De Swert 2, Charles Nicaise 2
1URVI, NARILIS, Université de Namur, 2URPhyM, NARILIS, Université de Namur
Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.
