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UCL Institute of Ophthalmology

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
Miguel Tillo 1, Quenten Schwarz 1,2, Christiana Ruhrberg 1
1UCL Institute of Ophthalmology, University College London, 2Department of Human Immunology, Centre for Cancer Biology, South Australia

Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.

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Neuroscience

The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
Mathew Tata 1, Christiana Ruhrberg 1
1UCL Institute of Ophthalmology

This article demonstrates how the mouse embryonic hindbrain can be used as a model for studying developmental neurogenesis in both whole organ and tissue section preparations.

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Biology

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
James T. Brash 1, Christiana Ruhrberg 1, Alessandro Fantin 1
1UCL Institute of Ophthalmology, University College London

Here, we present a protocol to measure the vascular leakage induced by intradermal administration of permeability promoting agents into the murine skin. This technique can be used to determine the ability of molecules to promote or inhibit vascular leakage or to study the molecular mechanisms that regulate vascular permeability.

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Medicine

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
Ivan Seah *1,2, Zengping Liu *1,3,4, Daniel Soo Lin Wong 1, Wendy Wong 2, Graham E. Holder 1,2,5, Veluchamy Amutha Barathi 1,4,6, Gopal Lingam 1,2,4, Xinyi Su 1,2,3,4, Boris V. Stanzel 1,7,8
1Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Ophthalmology, National University Hospital, Singapore, 3Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 4Singapore Eye Research Institute (SERI), 5UCL Institute of Ophthalmology, 6Academic Clinical Program in Ophthalmology, Duke-NUS Medical School, 7Macula Center Saar, Eye Clinic Sulzbach, Knappschaft Hospital Saar, 8Department of Ophthalmology, University of Bonn

The non-human primate (NHP) is an ideal model for studying human retinal cellular therapeutics due to the anatomical and genetic similarities. This manuscript describes a method for submacular transplantation of retinal pigment epithelial cells in the NHP eye and strategies to prevent intraoperative complications associated with macular manipulation.

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