The University of Alabama

2 ARTICLES PUBLISHED IN JoVE

Genetics

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Saman Saeidi^*1, Michael R. McKain^*1,2, Elizabeth A. Kellogg¹

¹Donald Danforth Plant Science Center, ²Department of Biological Sciences, The University of Alabama

This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.

Biochemistry

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Zekiye Ceren Arituluk^1,2, Bishnu Adhikari¹, Urmila Maitra¹, Caroline Goodman¹, Lukasz M. Ciesla¹

¹Department of Biological Sciences, The University of Alabama, ²Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.