The University of Alabama
2 ARTICLES PUBLISHED IN JoVE
Genetics
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Saman Saeidi *1, Michael R. McKain *1,2, Elizabeth A. Kellogg 1
1Donald Danforth Plant Science Center, 2Department of Biological Sciences, The University of Alabama
This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.
Biochemistry
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Zekiye Ceren Arituluk 1,2, Bishnu Adhikari 1, Urmila Maitra 1, Caroline Goodman 1, Lukasz M. Ciesla 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University
The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.
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