Humanitas Clinical and Research Center, Rozzano, Milan, Italy

2 ARTICLES PUBLISHED IN JoVE

Genetics

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Oriana Romano^1,2, Ingrid Cifola³, Valentina Poletti⁴, Marco Severgnini³, Clelia Peano³, Gianluca De Bellis³, Fulvio Mavilio^1,4, Annarita Miccio^1,2,4,5

¹Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, ²Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, ³Institute for Biomedical Technologies, CNR, ⁴Généthon, ⁵Sorbonne Paris Cité - Université Paris Descartes

Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.

Biology

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Maria Felicia Soluri¹, Simone Puccio², Giada Caredda², Giorgio Grillo³, Vito Flavio Licciulli³, Arianna Consiglio³, Paolo Edomi⁴, Claudio Santoro¹, Daniele Sblattero⁴, Clelia Peano^5,6

¹Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, ²Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, ³Institute of Biomedical Technologies, National Research Council, Bari, Italy, ⁴Department of Life Sciences, University of Trieste, Italy, ⁵Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, ⁶Humanitas Clinical and Research Center, Rozzano, Milan, Italy

The protocols described allow the construction, characterization and selection (against the target of choice) of a "domainome" library made from any DNA source. This is achieved by a research pipeline that combines different technologies: phage display, a folding reporter and next generation sequencing with a web tool for data analysis.