Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
8.8K Views
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12:04 min
•
October 3rd, 2018
DOI :
October 3rd, 2018
•Maria Felicia Soluri1, Simone Puccio2, Giada Caredda2, Giorgio Grillo3, Vito Flavio Licciulli3, Arianna Consiglio3, Paolo Edomi4, Claudio Santoro1, Daniele Sblattero4, Clelia Peano5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy
Chapters in this video
0:04
Title
1:19
Construction of the ORF Library
2:38
Preparation of the Filtering Vector
3:40
Ligation and Transformation
6:47
pFILTER-ORF Library Validation and Collection
9:10
Phage Library Deep Sequencing Platform, Index PCR Followed by Qualitative and Quantitative Evaluation of the Sequencing Library
10:07
Results: Use of a Phagemid Vector to Construct, Characterize and Select a Domainome Library
11:23
Conclusion
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