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Universitat de València

4 ARTICLES PUBLISHED IN JoVE

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Behavior

Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm
M. Carmen Blanco-Gandía 1, María A. Aguilar 1, José Miñarro 1, Marta Rodríguez-Arias 1
1Unidad de Investigación Psicobiología de las Drogodependencias, Departamento de Psicobiología, Facultad de Psicología, Universitat de València

This protocol describes the Conditioned Place Preference (CPP) as a model of relapse. This procedure permits the measurement of relapse in laboratory animals, considering the impact of drug-associated environmental cues as craving and relapse in abstaining addicts is currently the focus of drug-abuse treatment programs.

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Medicine

An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment
Óscar Julián Arias-Mutis 1,2,3, Patricia Genovés 1,2,3, Conrado J. Calvo 1,2,4, Ana Díaz 5, Germán Parra 2,3, Luis Such-Miquel 6, Luis Such 2, Antonio Alberola 2, Francisco Javier Chorro 1,3, Manuel Zarzoso 6
1CIBERCV, Instituto de Salud Carlos III, 2Department of Physiology, Universitat de València, 3INCLIVA, 4Department of Electronic Engineering, Universidad Politécnica de Valencia, 5UCIM, Universitat de València, 6Department of Physiotherapy, Universitat de València

We describe methods to develop an experimental model of diet-induced metabolic syndrome (MetS) in rabbits using a high-fat, high-sucrose diet. Animals developed central obesity, mild hypertension, pre-diabetes, and dyslipidemia, thus reproducing the main components of human MetS. This chronic model will allow acquisition of knowledge underlying mechanisms of disease progression.

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Chemistry

Surface Functionalization of Metal-Organic Frameworks for Improved Moisture Resistance
Javier Castells-Gil 1, Fernando Novio 2, Natalia M. Padial 1, Sergio Tatay 1, Daniel Ruíz-Molina 2, Carlos Martí-Gastaldo 1
1Instituto de Ciencia Molecular (ICMol), Universitat de València, 2Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and The Barcelona Institute of Science and Technology

Robust functional catechol coatings were produced in one step by direct reaction of the material known as HKUST with synthetic catechols under anaerobic conditions. The formation of homogeneous coatings surrounding the entire crystal is ascribed to the biomimetic catalytic activity of Cu(II) dimers on the external surface of the crystals.

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Neuroscience

Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone
Esteban Jiménez-Villalba *1, Laura Lázaro-Carot *1, Carmen M. Mateos-Martínez 1, Jennifer Díaz-Moncho 1, Daniel Samper-Llavador 1, Marta Igual-López 1, Jordi Planells 1, Sacri R. Ferrón 1
1Instituto de Biotecnología y Biomedicina (BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València

Here, we describe a nucleofection system designed to enhance gene delivery efficiency in expanded neural stem cells (NSCs) isolated from the adult murine subventricular zone. The findings demonstrate that this method significantly improves gene perturbation in NSCs, surpassing the effectiveness of traditional transfection protocols and enhancing cell survival rate.

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