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Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone

2.3K Views

09:19 min

June 14th, 2024

DOI :

10.3791/66651-v

June 14th, 2024


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Neural Stem Cells

Chapters in this video

0:00

Introduction

1:35

Murine Brain Extraction and Subventricular Zone (SVZ) Dissection for Isolation of Neural Stem Cells

3:42

Isolation and Seeding of Murine Subventricular Zone‐Derived Neural Stem Cells (NSCs) for Neurosphere Formation

5:54

Expansion of Neurospheres Obtained from Murine Subventricular Zone‐Derived Neural Stem Cells

7:33

Nucleofection of Neurosphere Cultures Obtained from Murine Subventricular Zone‐Derived Neural Stem Cells

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