Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone
2.2K Views
•
09:19 min
•
June 14th, 2024
DOI :
June 14th, 2024
•Chapters in this video
0:00
Introduction
1:35
Murine Brain Extraction and Subventricular Zone (SVZ) Dissection for Isolation of Neural Stem Cells
3:42
Isolation and Seeding of Murine Subventricular Zone‐Derived Neural Stem Cells (NSCs) for Neurosphere Formation
5:54
Expansion of Neurospheres Obtained from Murine Subventricular Zone‐Derived Neural Stem Cells
7:33
Nucleofection of Neurosphere Cultures Obtained from Murine Subventricular Zone‐Derived Neural Stem Cells
Related Videos
14:33
The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow
41.9K Views
08:48
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
9.2K Views
13:21
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
27.7K Views
07:00
Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles
16.9K Views
09:10
Preparation of Acute Subventricular Zone Slices for Calcium Imaging
13.2K Views
19:45
Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
16.8K Views
08:06
Neonatal Subventricular Zone Electroporation
12.8K Views
09:24
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
15.2K Views
10:05
Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
14.3K Views
04:10
Isolation and Culturing of Adult Neural Stem Cells from the Mouse Subcallosal Zone
37 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved