Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)

3 ARTICLES PUBLISHED IN JoVE

Medicine

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

Nodir Madrahimov¹, Ruslan Natanov¹, Erin C. Boyle¹, Tobias Goecke¹, Ann-Kathrin Knöfel¹, Valentyna Irkha¹, Anna Solovieva², Klaus Höffler¹, Ulrich Maus³, Christian Kühn¹, Issam Ismail¹, Gregor Warnecke¹, Malakh-Lal Shrestha¹, Serghei Cebotari^*1, Axel Haverich^*1

¹Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, ²Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen, ³Department of Pneumology, Hannover Medical School

This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage.

Immunology and Infection

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Vanessa Neuhaus^*1, Olga Danov^*1, Sebastian Konzok¹, Helena Obernolte¹, Susann Dehmel¹, Peter Braubach², Danny Jonigk², Hans-Gerd Fieguth³, Patrick Zardo⁴, Gregor Warnecke⁴, Christian Martin⁵, Armin Braun^1,6, Katherina Sewald¹

¹Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, ²Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ³Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), ⁴Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ⁵Institute of Pharmacology and Toxicology, RWTH Aachen University, ⁶Institute for Immunology, Hannover Medical School

In view of the 3Rs principle, respiratory models as alternatives to animal studies are evolving. Especially for risk assessment of respiratory substances, there is a lack of appropriate assays. Here, we describe the use of human precision-cut lung slices for the assessment of airborne substances.

Medicine

Phase-Resolved Functional Lung MRI for Pulmonary Ventilation and Perfusion (V/Q) Assessment

Andreas Voskrebenzev^1,2, Filip Klimeš^1,2, Frank Wacker^1,2, Jens Vogel-Claussen^1,2

¹Institute for Diagnostic and Interventional Radiology, Hannover Medical School, ²Member of the German Center for Lung Research, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)

Here, we describe the implementation of phase-resolved functional lung MRI as a contrast-agent-free proton MR technique for the assessment of pulmonary ventilation and perfusion dynamics. Validated and applicable across different field strengths and age groups, it could enhance clinical decision-making in the future by aiding in disease quantification and therapy monitoring.