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The New York State Office for People with Developmental Disabilities

2 ARTICLES PUBLISHED IN JoVE

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Chemistry

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
Mohameedyaseen Syedbasha 1, Janina Linnik 1,2,3, Deanna Santer 4, Daire O'Shea 5, Khaled Barakat 4,6, Michael Joyce 4, Nina Khanna 7, D. Lorne Tyrrell 4, Michael Houghton 4, Adrian Egli 1,8
1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, 3Swiss Institute of Bioinformatics, 4Li Ka Shing Institute for Virology, University of Alberta, 5Regional Infectious Diseases Unit, University of Edinburgh, 6Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 7Infection Biology, Department of Biomedicine, University of Basel, 8Clinical Microbiology, University Hospital Basel

The presented protocols describe two enzyme-linked immunosorbent assay (ELISA) based techniques for the rapid investigation of ligand-receptor interactions: The first assay allows the determination of dissociation constant between ligand and receptor. The second assay enables a rapid screening of blocking peptides for ligand-receptor interactions.

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Developmental Biology

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
George Merz 1, Valerie Schwenk 1, Ruchit Shah 2, Carolyn Salafia 1,2, Phillip Necaise 2, Michael Joyce 1, Tom Villani 3, Michael Johnson 3, Nick Crider 3
1The Institute for Basic Research, The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

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