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Friedrich-Alexander University of Erlangen-Nuremberg

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Isolation of Human Islets from Partially Pancreatectomized Patients
Gregor Bötticher 1, Dorothèe Sturm 1, Florian Ehehalt 1, Klaus P. Knoch 2, Stephan Kersting 1, Robert Grützmann 1, Gustavo B. Baretton 3, Michele Solimena 2, Hans D. Saeger 1
1Department of GI-, Thorax- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology, Paul Langerhans Institute Dresden, 3Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden

The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

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Medicine

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
Dorothée Sturm 1,2, Lorella Marselli 3, Florian Ehehalt 1,2, Daniela Richter 1, Marius Distler 2, Stephan Kersting 1,2, Robert Grützmann 2, Krister Bokvist 4, Philippe Froguel 5, Robin Liechti 6, Anne Jörns 7, Paolo Meda 8, Gustavo Bruno Baretton 9, Hans-Detlev Saeger 2, Anke M. Schulte 10, Piero Marchetti 3, Michele Solimena 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

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Cancer Research

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
Elisabeth Naschberger 1, Daniela Regensburger 1, Clara Tenkerian 1, Melanie Langheinrich 2, Felix B. Engel 3, Carol Geppert 4, Arndt Hartmann 4, Robert Grützmann 2, Vera S. Schellerer *2, Michael Stürzl *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg

Tumor endothelial cells are important determinants of the tumor microenvironment and the course of the disease. Here, a protocol for the isolation of pure and viable endothelial cells from human colorectal carcinoma and normal colon to be used in drug testing and pathogenesis research is described.

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Medicine

Investigating Intestinal Barrier Breakdown in Living Organoids
Marco Bardenbacher 1, Barbara Ruder 2, Natalie Britzen-Laurent 1, Elisabeth Naschberger 1, Christoph Becker 2, Ralph Palmisano 3, Michael Stürzl *1, Philipp Tripal *1,3
1Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, 3Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg

Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved manner.

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