Chinese People's Liberation Army (PLA) General Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Application of End-to-end Anastomosis in Robotic Central Pancreatectomy

Rong Liu^*1, Zi-Zheng Wang^*1, Yuan-Xing Gao¹, Yong Xu¹

¹Department of Hepatobiliary and Pancreatic Surgical Oncology, Chinese People's Liberation Army (PLA) General Hospital

The robotic central pancreatectomy with end-to-end anastomosis is feasible and safe for tumor in the pancreatic neck and proximal portion of pancreatic body. The operative techniques of this operation are presented.

Neuroscience

Free-floating Immunostaining of Mouse Brains

Longlong Tu¹, Nan Zhang^1,2,3, Kristine M Conde¹, Jonathan C Bean¹, Chunmei Wang¹, Yong Xu^1,4

¹USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, ²Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, ⁴Department of Molecular and Cellular Biology, Baylor College of Medicine

This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.