JoVE Logo
Faculty Resource Center

Sign In

Free-floating Immunostaining of Mouse Brains

DOI :

10.3791/62876-v

October 7th, 2021

October 7th, 2021

10,629 Views

1USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 2Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology, Baylor College of Medicine

This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.

Tags

Immunostaining

-- Views

Related Videos

article

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

article

Ex vivo Culturing of Whole, Developing Drosophila Brains

article

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

article

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

article

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

article

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

article

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

article

Simultaneous Cryosectioning of Multiple Rodent Brains

article

Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

article

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved