Institut für Molekulare Zellbiologie

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Markus Horsthemke¹, Janine Wilden¹, Anne C. Bachg¹, Peter J. Hanley¹

Here we describe methods using spinning disk confocal microscopy to image single phagocytic events by mouse resident peritoneal macrophages. The protocols can be extended to other phagocytic cells.

Immunology and Infection

Time-lapse Imaging of Mouse Macrophage Chemotaxis

Esther van den Bos¹, Stefan Walbaum¹, Markus Horsthemke¹, Anne C. Bachg¹, Peter J. Hanley¹

¹Institut für Molekulare Zellbiologie

Here we describe methods using time-lapse, phase-contrast microscopy to image mouse resident peritoneal macrophages in a chemotactic complement C5a gradient. The protocols can be extended to other immune cells.