Institut für Molekulare Zellbiologie

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
Markus Horsthemke 1, Janine Wilden 1, Anne C. Bachg 1, Peter J. Hanley 1
1Institut für Molekulare Zellbiologie

Here we describe methods using spinning disk confocal microscopy to image single phagocytic events by mouse resident peritoneal macrophages. The protocols can be extended to other phagocytic cells.

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Immunology and Infection

Time-lapse Imaging of Mouse Macrophage Chemotaxis
Esther van den Bos 1, Stefan Walbaum 1, Markus Horsthemke 1, Anne C. Bachg 1, Peter J. Hanley 1
1Institut für Molekulare Zellbiologie

Here we describe methods using time-lapse, phase-contrast microscopy to image mouse resident peritoneal macrophages in a chemotactic complement C5a gradient. The protocols can be extended to other immune cells.

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