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Universidad San Sebastián

3 ARTICLES PUBLISHED IN JoVE

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Coculture System to Study Osteoblast and Osteoclast Interactions in Prostate Cancer Progression to Hormone Resistance and Metastasis to Bone
Romina F. Amigo 1,2, Macarena A. Fariña 1, Nicolás A. Zuñiga 1, Marcelo A. Villagran 3, Eileen M. McNerney 1, Sergio A. Onate 1
1Molecular Endocrinology and Oncology Laboratory, Departent of Medical Specialties, School of Medicine, Universidad de Concepción, 2School of Health Sciences, Universidad San Sebastián, 3Department of Biochemistry, Universidad Católica de la Santísima Concepción

Prostate cancer bone metastasis is reactivated by fibroblast growth factors and nuclear receptor interactions, together with autocrine/paracrine factors in tumor microenvironment. A coculture cell model using the transwell system of cocultured cells was developed to determine the genes, FGFs and FGF receptors, potentially involved in the progression to bone metastasis.

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Immunology and Infection

Studying Organelle Dynamics in B Cells During Immune Synapse Formation
Jorge Ibañez-Vega *1, Danitza Fuentes *1, Jonathan Lagos 1,2, Jorge Cancino 3, María Isabel Yuseff 1
1Laboratory of Immune Cell Biology, Department of Cellular and Molecular Biology, Pontificia Universidad Católica de Chile, 2Faculty of Medicine, Pontificia Universidad Católica de Chile, 3Centro de Investigaciones en Biología Celular y Biomedicina, Facultad de Ciencia, Universidad San Sebastián

Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.

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Neuroscience

Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
Daniela Elgueta 1, Ornella Chovar 1, Valentina Ugalde 1, Vanesa Sánchez-Guajardo 1, Alejandra Catenaccio 2, Felipe Court 2,3,4, Rodrigo Pacheco 1,5
1Laboratorio de Neuroinmunología, Fundación Ciencia & Vida, 2Center for Integrative Biology, Universidad Mayor, 3FONDAP Geroscience Center for Brain Health and Metabolism, 4Buck Institute for Research on Ageing, 5Facultad de Medicina y Ciencia, Universidad San Sebastián

This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease.

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