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Studying Organelle Dynamics in B Cells During Immune Synapse Formation

8.9K Views

15:39 min

June 1st, 2019

DOI :

10.3791/59621-v

June 1st, 2019


Transcript

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Organelle Dynamics

Chapters in this video

0:00

Title

1:16

B Cell Activation with Antigen-coated Beads

2:48

B Cell Activation on Antigen-coated Coverslips

3:51

Immunofluoresence and Image Analysis

4:30

Analysis of the Distribution of Organelles in B Cells Activated with Ag-coated Beads

7:42

Analysis of Cell Spreading and Distribution of Organelles in B Cells Activated on Ag-coated Coverslips

9:29

Isolation of Centrosome-enriched Fractions from Resting and Activated B Cells

11:23

Results

15:07

Conclusion

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