Unité de Pathogénomique Mycobactérienne

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Violaine Dubois^*1, Laura Laencina^*1, Anouchka Bories¹, Vincent Le Moigne¹, Alexandre Pawlik², Jean-Louis Herrmann¹, Fabienne Girard-Misguich¹

¹2I, UVSQ, INSERM UMR1173, Université Paris-Saclay, ²Institut Pasteur, Unité de Pathogénomique Mycobactérienne

Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.