Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in PhagocytesViolaine Dubois *1, Laura Laencina *1, Anouchka Bories 1, Vincent Le Moigne 1, Alexandre Pawlik 2, Jean-Louis Herrmann 1, Fabienne Girard-Misguich 1
12I, UVSQ, INSERM UMR1173, Université Paris-Saclay, 2Institut Pasteur, Unité de Pathogénomique Mycobactérienne
Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.
