JoVE | Peer Reviewed Scientific Video Journal

Developmental Biology

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Zhenan Liu¹, Jie Wen^1,2, Xue Leng¹, Qian Zhou¹, Changkuo Zhou³, Huaqiang Zhao¹, Xunwei Wu^1,2

¹Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, ²Suzhou Institute of Shandong University, ³Department of Urology, Qilu Hospital of Shandong University

Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

Biology

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Chang Liu¹, Shuangshuang Wang¹, Man Liu², Fuxiang Bai¹, Zhihong Chen³, Ping Wang⁴, Jun Mi^1,5, Xunwei Wu¹

¹Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ²Shijiazhuang Shimen Experimental School, ³Qilu Children's Hospital of Shandong University, ⁴Qilu Hospital of Shandong University, ⁵Shenzhen Research Institute of Shandong University

This paper reports that the addition of Y-27632 to TIVA medium can significantly increase the yield of melanocytes from adult skin tissues.

Bioengineering

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Zhiwei Xie^1,2,3, Jizhou Shi⁴, Min Zong³, Qiuping Xu¹, Chang Liu¹, Jie Wen¹, Qun Zhang¹, Panpan Liu¹, Guanyi Liu¹, Jing Guo^2,5, Xunwei Wu¹

¹Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ²Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ³Department of Stomatology, Shengli Oilfield Central Hospital, ⁴Department of Pediatric Surgery, Shengli Oilfield Central Hospital, ⁵Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.

Suzhou Institute of Shandong University

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Biology

Bioengineering