Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
4.9K Views
•
06:47 min
•
November 6th, 2021
DOI :
November 6th, 2021
•Zhiwei Xie1,2,3, Jizhou Shi4, Min Zong3, Qiuping Xu1, Chang Liu1, Jie Wen1, Qun Zhang1, Panpan Liu1, Guanyi Liu1, Jing Guo2,5, Xunwei Wu1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College
Chapters in this video
0:00
Introduction
0:49
Gingival Tissue Pretreatment
1:13
Digestion of Gingival Tissue
2:46
Cell Passage
3:51
Representative Results
6:18
Conclusion
Related Videos
09:47
Isolation, Processing and Analysis of Murine Gingival Cells
18.8K Views
06:17
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
10.0K Views
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
10.7K Views
08:03
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
8.8K Views
08:14
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
15.1K Views
08:06
Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
5.4K Views
06:28
Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
5.7K Views
04:07
Isolation of Epithelial Cells from Human Dental Follicle
2.8K Views
05:05
Isolation and Culture of Primary Human Mammary Epithelial Cells
790 Views
01:39
A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions
228 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved