Ulm University Medical Center

3 ARTICLES PUBLISHED IN JoVE

Medicine

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Melanie Haffner-Luntzer¹, Fabian Müller-Graf^1,4, Romano Matthys², Alireza Abaei³, René Jonas¹, Florian Gebhard⁴, Volker Rasche^*3, Anita Ignatius^*1

¹Institute of Orthopedic Research and Biomechanics, University Medical Center Ulm, ²RISystem, ³Core Facility Small Animal MRI, University Medical Center Ulm, ⁴Department of Traumatology, Hand-, Plastic-, and Reconstructive Surgery, University Medical Center Ulm

The evaluation of tissue development in the fracture callus during endochondral bone healing is essential to monitor the healing process. Here, we report the use of a magnetic resonance imaging (MRI)-compatible external fixator for the mouse femur to allow MRI scans during bone regeneration in mice.

Medicine

Improved Registration of 3D CT Angiography with X-ray Fluoroscopy for Image Fusion During Transcatheter Aortic Valve Implantation

Ina Vernikouskaya¹, Wolfgang Rottbauer¹, Julia Seeger¹, Birgid Gonska¹, Jochen Wöhrle^*1, Volker Rasche^*1

¹Department of Internal Medicine II - Cardiology, Ulm University Medical Center

The aim of this study was to improve the co-registration for image fusion (IF) of pre-interventional CT data with real-time x-ray (XR) fluoroscopy during transfemoral transcatheter aortic valve implantation (TAVI).

Immunology and Infection

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Nikaïa Smith^1,2,3,4, Nassima Bekaddour^2,3,4, Nicolas Leboulanger^4,5, Yolande Richard^*6, Jean-Philippe Herbeuval^*2,3,4

¹Institute of Molecular Virology, Ulm University Medical Center, ²CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, ³Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, ⁴Université Paris Descartes, Sorbonne Paris Cité, ⁵Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, ⁶Université de Paris, Institut Cochin

In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells from healthy humans undergoing partial surgical tonsillectomy to study innate immune responses upon activation, mimicking viral infection in mucosal tissues.