Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
7.4K Views
•
07:38 min
•
June 14th, 2020
DOI :
June 14th, 2020
•Nikaïa Smith1,2,3,4, Nassima Bekaddour2,3,4, Nicolas Leboulanger4,5, Yolande Richard*6, Jean-Philippe Herbeuval*2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
Chapters in this video
0:04
Introduction
0:44
Dissection of Human Tonsillar Tissue
1:58
Isolation of Tonsillar Mononuclear Cells (TMCs)
3:33
Isolation of the TMCs by Cell Density Gradient
4:42
Preparing Cells for Use or Freezing
5:14
Results: Phenotype, Immunological Response, and Viability
6:55
Conclusion
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