University of California, San Diego School of Medicine

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery, Harvard Medical School, ⁴Division of SCI Research, VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

Biology

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.

Neuroscience

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Richard B. McCosh¹, Michael J. Kreisman¹, Kellie M. Breen¹

¹Department of Reproductive Medicine, University of California, San Diego School of Medicine

Here we present a tail-tip blood sampling protocol for frequent sample collection in unrestrained mice. This method is useful for assessing patterns of pulsatile luteinizing hormone secretion and could be adapted for analysis of other circulating factors.

Developmental Biology

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Sandra L. Leibel^1,2,3, Rachael N. McVicar^2,3, Alicia M. Winquist^2,3, Evan Y. Snyder^1,2,3

¹Department of Pediatrics, University of California, San Diego School of Medicine, ²Sanford Consortium for Regenerative Medicine, ³Sanford Burnham Prebys Medical Discovery Institute

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.

Medicine

Building Affordable, Durable, Medium-Fidelity Ballistic Gel Phantoms for Ultrasound-Guided Nerve Block Training

Brennan P. Marsh-Armstrong^*1, John F. Ryan^*1, David J. Mariano¹, Preetham J. Suresh², Benjamin Supat³

¹University of California, San Diego School of Medicine, ²Department of Anesthesia, University of California, San Diego Health, ³Department of Emergency Medicine, University of California, San Diego Health

Here, we demonstrate the design and creation of four custom ballistic gelatin ultrasound phantoms for ultrasound-guided regional anesthesia training. We designed the phantoms using computer-aided design software, used 3D-printed models to create silicone molds, and then poured melted ballistic gel into the molds to create custom tissue layers.