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University of California, San Diego School of Medicine

6 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Xuejun H. Parsons 1,2, Yang D. Teng 3,4, James F. Parsons 1,2, Evan Y. Snyder 1,2,5, David B. Smotrich 1,2,6, Dennis A. Moore 1,2
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery, Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, 6La Jolla IVF

We have established a protocol for induction of neuroblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human neuronal progenitors and neuronal cell types in the developing CNS for neural repair.

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Biology

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Xuejun H. Parsons 1,2, Yang D. Teng 3,4, James F. Parsons 1,2, Evan Y. Snyder 1,2,5, David B. Smotrich 1,2,6, Dennis A. Moore 1,2
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery, Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, 6La Jolla IVF

We have established a protocol for induction of cardioblasts direct from pluripotent human embryonic stem cells maintained under defined conditions with small molecules, which enables derivation of a large supply of human cardiac progenitors and functional cardiomyocytes for cardiovascular repair.

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Neuroscience

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion
Richard B. McCosh 1, Michael J. Kreisman 1, Kellie M. Breen 1
1Department of Reproductive Medicine, University of California, San Diego School of Medicine

Here we present a tail-tip blood sampling protocol for frequent sample collection in unrestrained mice. This method is useful for assessing patterns of pulsatile luteinizing hormone secretion and could be adapted for analysis of other circulating factors.

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Developmental Biology

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
Sandra L. Leibel 1,2,3, Rachael N. McVicar 2,3, Alicia M. Winquist 2,3, Evan Y. Snyder 1,2,3
1Department of Pediatrics, University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.

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Medicine

Building Affordable, Durable, Medium-Fidelity Ballistic Gel Phantoms for Ultrasound-Guided Nerve Block Training
Brennan P. Marsh-Armstrong *1, John F. Ryan *1, David J. Mariano 1, Preetham J. Suresh 2, Benjamin Supat 3
1University of California, San Diego School of Medicine, 2Department of Anesthesia, University of California, San Diego Health, 3Department of Emergency Medicine, University of California, San Diego Health

Here, we demonstrate the design and creation of four custom ballistic gelatin ultrasound phantoms for ultrasound-guided regional anesthesia training. We designed the phantoms using computer-aided design software, used 3D-printed models to create silicone molds, and then poured melted ballistic gel into the molds to create custom tissue layers.

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Developmental Biology

iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease
Rachael N. McVicar 1,2, Emily Smith 1,2, Melina Melameka 1,2, Anne Bush 1,2, Grace Goetz 1,2, Gailan Constantino 1,2, Matangi Kumar 1,2, Elizabeth Kwong 1,2, Evan Y. Snyder 1,2, Sandra L. Leibel 1,2,3
1Sanford Consortium for Regenerative Medicine, 2Sanford Burnham Prebys Medical Discovery Institute, 3Department of Pediatrics, University of California, San Diego School of Medicine

This article describes the generation of a complex, multi-cellular airway barrier model composed of induced pluripotent stem cell (iPSC)-derived lung epithelium, mesenchyme, endothelial cells, and macrophages in an air-liquid interface culture.

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