Sanquin Research and Landsteiner Laboratory, The Netherlands

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Donata De Marco¹, Renske Taggenbrock^2,3, Rosa Crespo³, Wouter Koudstaal³, Elizabeth Ramsburg⁴, Adrian Apetri³

¹Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, ²Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, ³Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, ⁴Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.