JoVE Logo
Faculty Resource Center

Sign In

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

DOI :

10.3791/58576-v

7:18 min

November 9th, 2018

November 9th, 2018

7,894 Views

1Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center, Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery, Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.

Tags

Cell based Assay

-- Views

Related Videos

article

Laser Capture Microdissection of Drosophila Peripheral Neurons

article

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

article

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

article

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

article

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

article

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood

article

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

article

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

article

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

article

Obtaining Human Microglia from Adult Human Brain Tissue

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved