Using High Content Imaging to Quantify Target Engagement in Adherent CellsHanna Axelsson 1,2, Helena Almqvist 1,2, Brinton Seashore-Ludlow 1,3
1Chemical Biology Consortium Sweden, Science for Life Laboratory, Karolinska Institutet, Solna, 2Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna, 3Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, Stockholm
Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).
